Additional Files: Mitochondrial RNA processing in absence of tRNA punctuations in octocorals

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Creator/Author:
Shimpi, Gaurav

Vargas, Sergio

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Year of Publication:
2017
Faculty:
Faculty of Geosciences

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Abstract:
Background: Mitogenome diversity is staggering among early branching animals with respect to size, gene density and content, gene orders, and number of tRNA genes, especially in cnidarians. This last point is of special interest as tRNA cleavage drives the maturation of mitochondrial mRNAs and is a primary mechanism for mt-RNA processing in animals. Mitochondrial RNA processing in non-bilaterian metazoans, some of which possess a single tRNA gene in their mitogenomes, is essentially unstudied despite its importance in understanding the evolution of mitochondrial transcription in animals. Results: We characterized the mature mitochondrial mRNA transcripts in a species of the octocoral genus Sinularia (Alcyoniidae: Octocorallia), and defined precise boundaries of transcription units using different molecular methods. Most mt-mRNAs were polycistronic units containing two or three genes and 5' and/or 3' untranslated regions (UTRs) of varied length. The octocoral specific, mtDNA-encoded mismatch repair gene, mtMutS, was found to undergo alternative polyadenylation (APA), and exhibited differential expression of alternate transcripts suggesting a unique regulatory mechanism for this gene. In addition, a long noncoding RNA complementary to the ATP6 gene (lncATP6) potentially involved in antisense regulation was detected. Conclusions: Mt-mRNA processing in octocorals bearing a single mt-tRNA is complex. Considering the variety of mitogenome arrangements known in cnidarians, and in general among non-bilaterian metazoans, our findings provide a first glimpse into the complex mtDNA transcription, mt-mRNA processing, and regulation among early branching animals and represents a first step towards understanding its functional and evolutionary implications.
Keywords:
Mitochondrial RNA
Polycistronic transcripts
Alternative polyadenylation (APA)
Long noncoding RNA (lncRNA)
Mitogenome
Cnidaria
Octocorals
tRNA punctuation
Non-bilaterian
DDC:
570 Life sciences
590 Zoological sciences

Identifier

DOI:
https://doi.org/10.5282/ubm/data.101
lmUB:
3b78826f-5bf8-486f-9bb8-9a83d5011813

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